I will try to quickly respond to your questions that I quote:
1. Are you using the same bit depth, sample rate, & rendering on all of them? Using the same DAW, computer, operating system, cables, other hardware, etc.? A: The 16x08 and the GP-10 were recorded on the same computer and DAW with everything like sample and bit rates etc the same. The Apogee Duet is not windows compatible. That file was recorded on my friends mac and sent to me where I volume matched it.
2. Do you have any DSP/mixer functions enabled on any of the interfaces or in their control software? A: No, all of that off or bypassed. The can be no effects, processing, or normalization when doing comparisons like this. The recording were volume matched by ear and meter and no compression or limiting etc could be used.
-On the US-16x08, do you have the "MIXER TRUE BYPASS" on or off? If the mixer is enabled, do you have any of the effects (EQ/Compression) enabled? A: I have the latest firmware and driver/settings panel. I can't manage to get the 'Mixer True Bypass' to EVER be set to OFF. So it is always indicating 'ON'. I do seem to maybe hear that the mixer output is closer to real time than the reported approx 10m round trip when monitoring 'through' the DAW. I can listen to both the mixer and the DAW input , pan them, mix them etc so that way I can get some impression of time differences. I like the mixer it is simple but seems to do the basic thing. The meters are very laggy idk why. Overall the 16x08 seems an appropriate evolutionary step up from the US-1800 which had no mixer and insufficient direct monitoring -mono/no pan and you could not mix them, they were just always on you could only blend them all vs the computer output with that knob. (my US1800 died and this US16x08 is the replacement).
-I'm not sure if the Duet has any effects or not. I think it uses some sort of software limiter (Soft Limit?). Maybe that could saturate the signal a bit? A: We made sure to turn that off.
-With the GP-10, are you using the GK pickup at all? Also, do you have it set to DRY-GUITAR or one of the RE-GUITAR options? I wonder if it actually completely bypasses ALL processing before the signal makes it to the computer, or if it still gets colored by something in the path. A: The GK pickup is not being used, not connected. The unit is set to USB routing: 'DRY-GUITAR'. It does seem to bypass everything nicely.
3. Do you have a way to use the exact same audio source or a single re-amp recording to play into the instrument inputs on each interface? That way we could have an actual control sample to run through a spectrum analyser and visually see the differences. A: I think I understand the intent of your question, but that intention is misplaced for this testing scenario. While is best to compare the exact same source, we can't do that for these sorts of things. Maybe if we had a guitar-strumming robot ! lol. We MUST use an ACTUAL guitar for EACH recording, because we are concerned with how the passive pickup, high impedance guitar signals are recorded by each interface, for comparison of THAT. So we CAN'T use a pre-recorded single source file because that would be a 'line' level type thing. There would be no point, and it would be utterly confounding, and next to impossible, to find a way to somehow convert line level to the exact signal character of a passive guitar pickup. BTW you can 'sortof' do that sort of thing with mic by playing the signal through the same set of monitors the same distance away at same level etc and placing the mic in front of it. Yes that will give you a way to do a controlled comparison of mics or mic inputs. Though I say 'sortof' because of course the monitors are not the same as acoustic signals like voice, drums, acoustic guitar. Though that would be decent for comparing guitar amp speaker sound. This entire thread is because we are comparing guitar signals/loading, not line level input signals, not mic signals. I doubt there are any significant audible differences in line level inputs. I expect some differences in mic pres but that not the scope of this guitar input concern. The concept of using some kind of spectral graphic display is actually not so different across these recording vs is they were the same file. Use your ears and eyes. I havn't bothered trying to roughly name those, or maybe try to make the full range ones sound 'just like ' the duller ones with a single lowpass filter, which would then tell you something about the differences also in terms of about how much is 'missing'. The main concern is that they are so noticeably off, with all other reasonable conditions set to reasonable equivalence. I have emailed the files to Tascam, they say they will try to reproduce the concern. I hope it's something I missed somehow somewhere, that would be best. But it could be an actual issue. I hope we learn more about it soon.